Fig. 3

Proteomic profiling in BCG-treated NMIBC patient urine samples upon refrigeration. Comparison of total protein (a) and creatinine (b) concentrations between urine supernatant samples (n = 20) from BCG-treated NMIBC patients freshly frozen versus frozen after overnight refrigeration. A non-parametric paired t test (left panel, with no statistically significant differences) and a Spearman r test with two-tailed P-value (right panel) were performed. Each colour represents an individual patient. The dotted lines represent the minimum (Min NV) and maximum (Max NV) normal values in single void urine samples, serving as a reference control for our results. c, d Comparison of the urinary concentration of each detected soluble factor from BCG-treated NMIBC patients between freshly frozen versus frozen after overnight refrigeration. Analytes, namely IL-1b, IL-7, IL-17E/IL-25, IL-22, and PDGF-AB/BB, that were below the limit of detection or had paired samples from fewer than seven samples were not plotted. No significant differences were observed between measurements for each analyte in the group analysis using a mixed-effect model (REML) with a Sidak multiple comparisons test (c). Correlations between fresh and overnight refrigerated at 4℃ cell-free urine supernatants from BCG-treated non-muscle invasive bladder cancer patients for each measured analyte, and the Spearman rho test with two-tailed P test was calculated using the Python stats module of the SciPy library (d)